Identification of Trans-4-Hydroxy-L-Proline as a Compatible Solute and Its Biosynthesis and Molecular Characterization in Halobacillus halophilus
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منابع مشابه
Identification of Trans-4-Hydroxy-L-Proline as a Compatible Solute and Its Biosynthesis and Molecular Characterization in Halobacillus halophilus
Halobacillus halophilus, a moderately halophilic bacterium, accumulates a variety of compatible solutes including glycine betaine, glutamate, glutamine, proline, and ectoine to cope with osmotic stress. Non-targeted analysis of intracellular organic compounds using 1H-NMR showed that a large amount of trans-4-hydroxy-L-proline (Hyp), which has not been reported as a compatible solute in H. halo...
متن کاملIdentification and characterization of trans-3-hydroxy-l-proline dehydratase and Δ1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria
trans-4-Hydroxy-l-proline (T4LHyp) and trans-3-hydroxy-l-proline (T3LHyp) occur mainly in collagen. A few bacteria can convert T4LHyp to α-ketoglutarate, and we previously revealed a hypothetical pathway consisting of four enzymes at the molecular level (J Biol Chem (2007) 282, 6685-6695; J Biol Chem (2012) 287, 32674-32688). Here, we first found that Azospirillum brasilense has the ability to ...
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Halobacillus halophilus, a moderately halophilic bacterium isolated from salt marshes, produces various compatible solutes to cope with osmotic stress. Glutamate and glutamine are dominant compatible solutes at mild salinities. Glutamine synthetase activity in cell suspensions of Halobacillus halophilus wild type was shown to be salt dependent and chloride modulated. A possible candidate to cat...
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26 Gerhart, J. C., and A. B. Pardee, in Synthesis and Structure of Macromolecules, Cold Spring Harbor Symposia on Quantitative Biology, vol. 28 (1963), p. 491. 27 Changeux, J. P., in Subunit Structure of Proteins, Brookhaven Symposia in Biology, no. 17 (1964), p. 232. 28 Antonini, E., J. Wyman, M. Brunori, J. F. Taylor, A. Rossi-Fanelli, and A. Caputo, J. Biol. Chem., 239, 907 (1964). 29 Gibson...
متن کاملEpimerization of trans-4-hydroxy-L-proline to cis-4-hydroxy-D-proline during acid hydrolysis of collagen.
1. trans-4-Hydroxy-r,-proline is epimerized to &+hydroxy-n-proline under the conditions generally used for the hydrolysis of proteins for analyses of amino acids. 2. The cis epimer elutes from a column of UR-30 resin on a Beckman amino acid analyzer at the same time as threonine and hence would be undetected. 3. About 8% of the trans-4-hydroxy-L-proline in collagen can be epimerized to c&4-hydr...
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2017
ISSN: 1664-302X
DOI: 10.3389/fmicb.2017.02054